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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in lots of tumors, which leads to chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important part in advertising mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two added splice variants were being reported but thorough nucleotide sequence info is not still out there.
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Blocking peptides are peptides that bind precisely to the goal antibody and block antibody binding. These peptide normally contains the epitope regarded via the antibody.
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This guidebook outlines further more possible optimization of mobile and immunoassay parameters to be sure the absolute best outcomes are...
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This information outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies bound to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody compared to the antibody by yourself, you can see which staining is particular.
Concentrate on Information and facts Cyclin E belongs towards the remarkably conserved cyclin relatives, whose members are characterised by a dramatic periodicity in protein abundance in the cell cycle. Cyclins function as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation designs which contribute into the temporal coordination of every mitotic event. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S section boundary and is also degraded as cells progress by S period.
The measurement of protein phosphorylation is a useful website tool for measuring the modulation of receptor activation by both of those antibodies...
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